Background: Orthopedic wound infections pose a significant threat to patient health, particularly in low-resource settings. The emergence of extended-spectrum beta-lactamase (ESBL) producing bacteria has further complicated treatment options. Objectives: This study aimed to determine the prevalence of ESBL-producing bacteria in orthopedic wound infections in Kano, Nigeria, and to identify the most common ESBL-producing bacteria and their antimicrobial resistance patterns. Methods: A cross-sectional study was conducted at a five tertiary hospitals in Kano (National Orthopedic Hospital, Dala, Aminu Kano Teaching Hospital, Sir Muhammad Sunusi Specialist Hospital, Sheikh Jidda Specialist Hospital and Murtala Muhammad Specialist Hospital, Kano) Nigeria. Wound swab samples were collected from 400 orthopedic patients with suspected infections. Bacteria were isolated and identified using standard microbiological techniques. Extended Spectrum Beta lactamase (ESBL) producing isolates were confirmed using the double discs’ synergy test. Results: A total of 336 (84%) wound samples yielded bacterial growth; Gram negative bacteria were predominant with 258 (58.2%) isolates with 30 (22.1%) samples positive for ESBL-producing bacteria. The most common ESBL-producing bacteria were Pseudomonas aeruginosa 123(27.8%), Klebsiella pneumoniae 30(6.8%) and Escherichia coli 11(2.5%). High levels of resistance to third-generation cephalosporins, fluoroquinolones, and aminoglycosides were observed. Conclusion: This study highlights the high prevalence of ESBL-producing bacteria in orthopedic wound infections in Kano, Nigeria. The findings emphasize the need for effective infection control measures, antimicrobial stewardship programs, and regular surveillance of ESBL-producing bacteria to combat the spread of antibiotic resistance.