Access to adequate clean water is a global challenge, particularly in developing countries where rural populations rely on water from rivers, dams and streams that may contain pathogenic and toxic materials. This research investigates the effect of Moringa oleifera seeds as a natural coagulant in treatment of water. The experimental procedure involved preparing M. oleifera seeds solution at varying concentrations, identifying the optimal dosage (120 mg/L) based on the earliest flocculation and sedimentation after 30 minutes. Physico-chemical parameters were analyzed using standard methods, and data from Atomic Absorption Spectroscopy (AAS) were analyzed using t-test with a significance level of p≤0.01. Two-way ANOVA was applied to assess significant differences (p˂0.05) among the physico-chemical parameters. The results show significant reductions in electrical conductivity from (54.4µs/cm to 53.4µs/cm), turbidity from (11.34 NTU to 8.45 NTU), total suspended solids (TSS) from (0.867 mg/L to 0.821 mg/L), total dissolved solids (TDS) from (198.6 mg/L to 196.3 mg/L), and chloride levels from (178.54 mg/L to 167.34 mg/L). The treatment also demonstrates selective effects on metal levels, significantly increasing Chromium concentrations (0.06±0.01) to (0.27±0.21), Nickel concentrations (0.38±0.02) to (0.48±0.02) while significantly reducing Copper levels (0.03±0.20) to (0.013±0.00) and having no significant effect on Cadmium and Cobalt levels. These findings suggest that the treatment process is effective in reducing heavy metal concentration in contaminated water and improving water quality. Further studies should explore the effectiveness of M. oleifera in combination with other coagulants to enhance its ability to remove broader range of heavy metals.